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Diversity patterns and activity of uncultured marine heterotrophic flagellates unveiled with pyrosequencing ArchiMer
Logares, Ramiro; Audic, Stephane; Santini, Sebastien; Pernice, Massimo C.; De Vargas, Colomban; Massana, Ramon.
Flagellated heterotrophic microeukaryotes have key roles for the functioning of marine ecosystems as they channel large amounts of organic carbon to the upper trophic levels and control the population sizes of bacteria and archaea. Still, we know very little on the diversity patterns of most groups constituting this evolutionary heterogeneous assemblage. Here, we investigate 11 groups of uncultured flagellates known as MArine STramenopiles (MASTs). MASTs are ecologically very important and branch at the base of stramenopiles. We explored the diversity patterns of MASTs using pyrosequencing (18S rDNA) in coastal European waters. We found that MAST groups range from highly to lowly diversified. Pyrosequencing (hereafter '454') allowed us to approach to the...
Tipo: Text Palavras-chave: Diversity; Heterotrophic-flagellates; MAST; Pyrosequencing; Stramenopiles.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00374/48501/48815.pdf
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